There have been more confirmed detections in the Japanese encephalitis outbreak around Australia.
A second person in Queensland has tested positive to mosquito-borne JEV while another two are listed as suspected cases.
A total of 20 people are confirmed as having the virus with a further 12 people in Queensland, New South Wales, South Australia and Victoria listed as probable cases.
NSW has eight confirmed cases, Victoria has seven, South Australia three and Queensland two.
Three people have died from JEV - one each in NSW, Victoria and South Australia.
There have been three more JEV detections in South Australian piggeries, bringing the total number of cases in SA to four.
The detections are in the local government areas of Loxton, Waikerie, Murray Bridge, and Coorong.
The virus has been detected at one new piggery in Victoria.
Detections have been confirmed at a total of 15 properties in the Wangaratta, Moira, Greater Shepparton, Campaspe, Gannawarra, Loddon, Greater Bendigo and Northern Grampians local government areas.
In NSW, healthy authorities say confirmed human cases have "potentially been exposed" in Balranald, Berrigan, Federation, Goulburn Mulwaree, Griffith, Temora and Wentworth.
Many suspected cases are under investigation in humans and piggeries around the country.
South Australia's chief veterinary officer Dr Mary Carr said the message was clear: prevent Japanese encephalitis in pigs and horses by controlling mosquitoes and protecting your animals from mosquito bites.
"PIRSA urges all pig and horse owners to reduce the risk by controlling mosquitoes on your property, through eliminating breeding areas and using chemicals responsibly," Dr Carr said.
"Prevent mosquitoes biting pigs and horses and protect yourself, your staff and your family."
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Pork SA chair Andrew Johnson said the industry was working closely with PIRSA and SA Health.
"We welcome the prompt response from the state government on this issue of JE, with information coming to industry even before detections were confirmed in South Australia," Mr Johnson said.
"There are staff on the ground conducting mosquito and disease surveillance, including further testing and providing information and advice to farmers.
"There are no food safety issues associated with eating pork meat or pork products due to this disease. Shoppers are encouraged to support local farmers by continuing to buy Australian pork.
Agriculture Victoria said it was also working closely with both the pig and horse industries in response to the disease. A number of suspected cases are under investigation.
Vaccines are recommended for people at a higher risk of exposure to the virus, such as those working with pigs.
Priorities have been set for the rollout of vaccines to help prevent more fatalities from the Japanese encephalitis outbreak.
Pigs are known to "amplify" the disease.
The federal government is spending $69 million on vaccines and mosquito management systems.
These first jabs will be supplied from the national stockpile of about 15,000 doses.
The government will spend almost $30 million buying more vaccines from across the world - enough for an extra 130,000 doses.
Health Minister Greg Hunt said those new vaccines would likely be secured over the next month.
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